Laboratory of Cell Pathology and Targeted Therapy
Laboratory Mission
Development of new approaches to increasing the effectiveness of targeted therapy and overcoming drug resistance in oncological diseases, primarily in non-small cell lung cancer.
Scientific direction
Lung cancer remains one of the leading causes of cancer death worldwide. Despite significant progress in the field of targeted therapy, the effectiveness of treatment is often limited by the development of resistance of tumor cells to antitumor drugs. Studying the mechanisms of the formation of drug resistance and finding ways to overcome it are among the key tasks of modern oncology.
Laboratory research is focused on the study of molecular mechanisms of tumor growth and resistance to therapy, as well as on the development of new combined approaches to the treatment of non-small cell lung cancer. Special attention is paid to the search for compounds capable of enhancing the action of target drugs, to the study of the role of mitochondria in the survival and death of tumor cells, and to the development of new methods of selective impact on tumor tissue.
To solve these problems, modern experimental models are used, including three-dimensional cell cultures, organoid systems and tumor models in vivo. One of the promising directions is the use of pulse-field ablation in combination with drug therapy to increase the effectiveness of antitumor treatment.
General tasks
Study of drug resistance of tumor cells.
Search for new therapeutic targets and potential antitumor compounds.
Investigation of the role of mitochondria and cell metabolism in the response of tumor cells to therapy.
Development and optimization of combined approaches, including targeted therapy and physical methods of impact on tumors.
Evaluation of the effectiveness of new therapeutic strategies in cellular, organoid and animal models.
Methods
The laboratory uses a wide range of modern methods of cell and molecular biology, including flow cytometry, confocal and electron microscopy, gene and protein expression analysis, assessment of cell metabolism and viability, as well as genome editing technologies.
Equipment
The laboratory is equipped with modern equipment for conducting research in the field of cellular and molecular biology. The infrastructure allows you to perform a full cycle of work — from cell cultivation and conducting experiments on animal models to molecular analysis and high-resolution microscopy. Key features include working with cell cultures, gene and protein analysis, fluorescent, confocal and electron microscopy.
Grants
2025-2030 "Integration grant 2025/2", Committee of higher education and science RA (research project 25IRF/2-1F004).
Publications
1. Fedotov SA, Stepanov AV, Sakuta GA, Vorobev ML, Baidyuk EV. Functional changes in mitochondrial subpopulations of left ventricular cardiomyocytes in post-infarction rats during the subacute stage of remodeling. Front. Biosci. (Landmark Ed), 2026. 31(6): 47046. https://doi.org/10.31083/FBL47046
2. Fedotov SA, Belashova TA, Kulichikhin KY, Glotov AS, Rubel AA. Assessing amyloidogenic potential of urinary proteins from women with preeclampsia using yeast-based assay // Ecological genetics, 2026. 24(1): 65-72. https://doi.org/10.17816/ecogen698555
3. Fedotov SA, Goncharova AA, Besedina NG, Danilenkova LV, Kamysheva EA, Solodukhina UN, Rubel AA, Bragina JV. The structure of courtship behavior in Drosophila males: Boundaries of plasticity. Behavioural Processes, 2026. 234: 105312. https://doi.org/10.1016/j.beproc.2025.105312
4. Fedotov SA, Stepanov AV, Sakuta GA, Andreev IS, Ivanova MS, Baidyuk EV. Ultrastructural Remodeling of Cardiomyocytes in Postinfarction Myocardium of Rats in the Late Stages of the Disease. Cytometry Part A, 2025. 107:36-44. https://doi.org/10.1002/cyto.a.24915
5. Leonova EI, Chirinskaite AV, Akhmarov II, Luganskaya PS, Kirillov OA, Kandina DA, Romanovich AE, Shkodenko LA, Fedotov SA, Rubel AA, Sopova YV. Improved genome editing via oviductal nucleic acids delivery-based in vivo electroporation technique for knockout mice generation. J. Vis. Exp., 2025. 222: e68704. https://doi.org/10.3791/68704
6. Bragina JV, Danilenkova LV, Kamysheva EA, Goncharova AA, Fedotov SA, Larina ON, Burlakova AA, Besedina NG. Behavioral changes in Drosophila males after travel to International Space Station. Part II. Larvae vs. Imago. Acta Astronautica, 2025. 229: 192-198. https://doi.org/10.1016/j.actaastro.2025.01.025
7. Gabrielyan Y, Hunanyan A, Bezirganyan S, Davtyan L, Avetisyan A et al. Comparative Analysis of Deep Learning Methods for Classification of Ablated Regions in Hyperspectral Images of Atrial Tissue. IEEE Access, 2025. 13, pp:35029-35047. https://doi.org/10.1109/ACCESS.2025.3543122
8. Fedotov SA, Khrabrova MS, Vashukova ES, Glotov AS, Anpilova AO, Dobronravov VA, Velizhanina ME, Rubel AA. Diagnostics of preeclampsia based on Congo red binding to urinary components: Rationales and limitations. PLoS ONE, 2024. 19(1): e0297144. https://doi.org/10.1371/journal.pone.0297144
9. Piletsky SS, Baidyuk E, Piletska EV, et al. Modulation of EGFR Activity by Molecularly Imprinted Polymer Nanoparticles Targeting Intracellular Epitopes. Nano Lett. 2023;23(21):9677-9682. https://doi.org/10.1021/acs.nanolett.3c01374
10. Fedotov SA, Baidyuk EV. Communication as the Origin of Consciousness. Integrative Psychological and Behavioral Science. 2023;57(1):20–42. https://doi.org/10.1007/s12124-022-09686-4
11. Goncharova AA, Besedina NG, Bragina JV, Danilenkova LV, Kamysheva EA, Fedotov SA. Courtship suppression in Drosophila melanogaster: The role of mating failure. PLoS ONE, 2023. 18(8):e0290048. https://doi.org/10.1371/journal.pone.0290048
12. Kachkin DV, Lashkul VV, Gorsheneva NA, Fedotov SA, Rubel MS, Chernoff YO, Rubel AA. The Aβ42 Peptide and IAPP Physically Interact in a Yeast-Based Assay. Int. J. Mol. Sci., 2023. 24(18): 14122. https://doi.org/10.3390/ijms241814122
13. Zelepukin IV, Griaznova OY, Shevchenko KG, Ivanov AV, Baidyuk EV, et al. Flash drug release from nanoparticles accumulated in the targeted blood vessels facilitates the tumour treatment. Nat Commun. 2022;13(1):6910. Published 2022 Nov 14. https://doi.org/10.1038/s41467-022-34718-3
14. Fedotov SA, Khrabrova MS, Anpilova AO, Dobronravov VA, Rubel AA. Noninvasive Diagnostics of Renal Amyloidosis: Current State and Perspectives. Int. J. Mol. Sci., 2022. 23(20):12662. https://doi.org/10.3390/ijms232012662
15. Kachkin DV, Volkov KV, Sopova JV, Bobylev AG, Fedotov SA, Inge-Vechtomov SG, Galzitskaya OV, Chernoff YO, Rubel AA, Aksenova AY. Human RAD51 protein forms amyloid-like aggregates in vitro. Int. J. Mol. Sci., 2022. 23(19):11657. https://doi.org/10.3390/ijms231911657
16. Baidyuk EV, Belotserkovskaya EV, Girshova LL, Golotin VA, Levchuk KA, Vasyutina ML, et al. Acute Myeloid Leukemia Patient-Derived Xenograft Models Generated with the Use of Immunodeficient NSG-SGM3 Mice. Clinical oncohematology. 2021;14(4):414–25. https://doi.org/10.21320/2500-2139-2021-14-4-414-425
17. Uyanik B, Goloudina AR, Akbarali A, Grigorash BB, Petukhov AV, Singhal S, Eruslanov E, Chaloyard J, Lagorgette L, Hadi T, Baidyuk EV, et al. Inhibition of the DNA damage response phosphatase PPM1D reprograms neutrophils to enhance anti-tumor immune responses. Nat Commun. 2021;12(1):3622. Published 2021 Jun 15. https://doi.org/10.1038/s41467-021-23330-6
18. Daks A, Shuvalov O, Fedorova O, Petukhov A, Lezina L, Zharova A, Baidyuk E, et al. p53-Independent Effects of Set7/9 Lysine Methyltransferase on Metabolism of Non-Small Cell Lung Cancer Cells. Front Oncol. 2021;11:706668. Published 2021 Oct 6. https://doi.org/10.3389/fonc.2021.706668
19. Baidyuk E, Vasyutina M, Girshova L, Petukhov A, Zaritsky A, et al. Establishment of cell line xenografts and PDX models for studying autophagy and apoptosis in response to chemotherapy in AML. Cell Death Discovery. 2021; Sep 1;7(Suppl 1):1–14. https://doi.org/10.1038/s41420-021-00587-w
20. Fedorova O, Gudovich A, Daks A, Baidyuk E, et al. Regulation of autophagy flux by E3 ubiquitin ligase Pirh2 in lung cancer. Biochem Biophys Res Commun. 2021;563:119-125. https://doi.org/10.1016/j.bbrc.2021.05.024
21. Kulichikhin KY, Fedotov SA, Rubel MS, Zalutskaya NM, Zobnina AE, Malikova OA, Neznanov NG, Chernoff YO, Rubel AA. Development of molecular tools for diagnosis of Alzheimer's disease that are based on detection of amyloidogenic proteins. Prion, 2021. 15(1):56-69. https://doi.org/10.1080/19336896.2021.1917289
22. Baidyuk E, Shuvalov OY, Semenov OM, Daks AA, Fedorova OA, et al. Autophagy level in lung adenocarcinoma cells with different EGFR status. Selected Conference Abstracts from “The Regulation of Proteostasis in Cancer”. Cell Death Discov. 2020;6 (Suppl 1), 1–22. https://doi.org/10.1038/s41420-020-0248-5
23. Baidyuk E. The role of autophagy in tyrosine kinase inhibitors treatment of lung cancer depending on the presence of different EGFR gene mutations. Abstracts. Eur J Clin Invest. 2020;50 (Issue S1): e13369. https://doi.org/10.1111/eci.13369
24. Baidyuk EV, Sobolev VE, Korf EA, Mindukshev IV, Krivchenko AI, Goncharov NV. Ultrastructural Characteristics of Slow and fast twitch Muscle Fibers of Rats after a Forced Swimming Cycle against a Background of Introduction of Green Tea Extract and Ammonium Salts. Cell and Tissue Biology. 2020;14(1):65–73. https://doi.org/10.1134/S1990519X20010022
25. Rubel MS, Fedotov SA, Grizel AV, Sopova JV, Malikova OA, Chernoff YO, Rubel AA. Functional mammalian amyloids and amyloid-like proteins. Life, 2020. 10(9):156. https://doi.org/10.3390/life10090156
26. Kamyshev NG, Besedina NG, Bragina JV, Danilenkova LV, Fedotov SA, Goncharova AA, Kamysheva EA, Burlakova AA, Larina ON. Behavioral changes in Drosophila males after travel to international space station. Acta Astronautica, 2020. 176:567-575 https://doi.org/10.1016/j.actaastro.2020.06.048